Intra-and interspecific protoplast fusion between some Pleurotus species.

Strain Improvement of Pleurotus Species by Protoplast Fusion

Objective: To improve the strain of Pleurotus mushroom by protoplast fusion technique Methods: The white rot fungus Pleurotus species was isolated from fresh fruiting body of Oyster mushroom by spore print technique and was cultured on Potato dextrose agar. The selected strain was identified based on the morphological and cultural characters on Potato dextrose agar. The protoplasts were isolate...

Protoplast Electrofusion for Development of Somatic Hybrids between Pleurotus Florida and Pleurotus Sajor-caju

Interspecific hybrids of Pleurotus florida PF-5 (MTCC 1801) and Pleurotus sajor-caju PSC3 (ATCC 62887) were developed using electrofusion. A concentration of 2mM vanillin and 1000ppm carbendazim were selected as screening markers for the hybrids of the two Pleurotus spp. The electrofusion using an exponential decay was best with 1000 V (2 pulses with an interval of 5 sec). With square wave disc...

Interspecific complementation analysis by protoplast fusion of Candida tropicalis and Candida albicans adenine auxotrophs.

A protocol employing inositol starvation was used to isolate proline and adenine auxotrophs of Candida tropicalis. Interspecific hybrids between red adenine auxotrophs of C. tropicalis and Candida albicans were formed by protoplast fusion. These C. tropicalis red adenine auxotrophs were shown to fall into two complementation groups by crossing them with a known C. albicans ade1 tester strain. I...

Protoplast Culture and Fusion between Brassica Carinata and Brassica Napus

In this study, attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines (BC DH Dodolla, BC DH -1, BC DH-6 and BN OP-1, BN-SL03/04), obtained from our previous experiments. Combination of three different PEG concentrations (20%, 25% or 30%) and two different treatment durations (15 and 20 min.) were te...

Budding Compatibility of Almond and Plum on Some Wild Species and Interspecific Hybrids of Almond